Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (HA1-V5229) is expressed from human 293 cells (HEK293). It contains AA Asp 17 - Gln 531 (Accession # AAC32099.1).
Predicted N-terminus: Asp 17
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.1 kDa. The protein migrates as 67-86 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229) was more than 90% and the molecular weight of this protein is around 95-110 kDa verified by SEC-MALS.
Immobilized Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza HA Universal Antibody, Human IgG1 with a linear range of 2-78 ng/mL (QC tested).
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.