登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Glycoprotein / GP (virus) >BEP-V5221

Ebolavirus (subtype Bundibugyo, strain Uganda 2007) Envelope Glycoprotein 1 (GP1) Protein, His Tag

分子别名(Synonym)

GP,Envelope glycoprotein,GP(1,2)

表达区间及表达系统(Source)

Ebolavirus (subtype Bundibugyo, strain Uganda 2007) GP1 Protein, His Tag (BEP-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Gln 304 (Accession # B8XCN0 ).

Predicted N-terminus: Ile 33

Request for sequence

蛋白结构(Molecular Characterization)

Glycoprotein / GP (virus) Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Glycoprotein / GP (virus) SDS-PAGE

Ebolavirus (subtype Bundibugyo, strain Uganda 2007) GP1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

背景(Background)

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

文献引用(Citations)

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Glycoprotein / GP (virus)靶点信息
英文全称:Envelope glycoprotein
中文全称:包膜糖蛋白
种类:
上市药物数量:2详情
临床药物数量:2详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定