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Mouse IL-23 R Protein, Fc Tag

分子别名(Synonym)

IL-23 R,IL-23 Receptor

表达区间及表达系统(Source)

Mouse IL-23R, Fc Tag (ILR-M5251) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Asp 372 (Accession # Q8K4B4-1).

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 24 - Asp 372) Q8K4B4-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 66.8 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse IL-23R, Fc Tag (Cat. No. ILR-M5251) SDS-PAGE gel

Mouse IL-23R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse IL-23R, Fc Tag (Cat. No. ILR-M5251) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 23.1 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

Interleukin 23 receptor (IL-23R) is a type I cytokine receptor, and IL-23R pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. Also, IL-23R associates constitutively with Janus kinase 2 (JAK2), and binds to transcription activator STAT3 in a ligand-dependent manner. Furthermore, IL-23R mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. As for IL-23, it may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

 

前沿进展

 
 
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IL-23R靶点信息
英文全称:Interleukin 23 Receptor
中文全称:白细胞介素23受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床三期
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