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 >  Protein>IgG1 Fc >IG1-M5208

Mouse IgG1 Fc Protein, Tag Free (MALS verified)

分子别名(Synonym)

IgG1

表达区间及表达系统(Source)

Mouse IgG Fc, Tag Free (IG1-M5208) is expressed from human 293 cells (HEK293). It contains AA Val 111 - Lys 324 (Accession # AAK53870.1).

Predicted N-terminus: Val 111

Request for sequence

蛋白结构(Molecular Characterization)

Online(Val 111 - Lys 324) AAK53870.1

This protein carries no "tag".

The protein has a calculated MW of 24.3 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) SDS-PAGE gel

Mouse IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) MALS images

The purity of Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) is more than 95% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) on NTA Biosensor, can bind Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) with an affinity constant of 17.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

 

 

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