B7-H3,CD276,B7 homolog 3
Mouse B7-H3, Fc Tag (B73-M5255) is expressed from human 293 cells (HEK293). It contains AA Val 29 - Phe 244 (Accession # NP_598744).
Predicted N-terminus: Val 29
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse B7-H3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse B7-H3, Fc Tag (Cat. No. B73-M5255) was more than 90% and the molecular weight of this protein is around 110-130 kDa verified by SEC-MALS.
Immobilized Mouse B7-H3, Fc Tag (Cat. No. B73-M5255) at 1 μg/mL (100 μL/well) can bind Anti-B7-H3 Antibody, Mouse IgG1 with a linear range of 4-63 ng/mL (QC tested).
Authors: Du H, et al
Journal: Cancer Cell 2019
Application: Stimulation of B7-H3 CAR-Ts
B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.