Recombinant Proteins iso9001 ISO13485
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Human VCAM-1 / CD106 Protein, His Tag

分子别名(Synonym)

VCAM1,CD106,INCAM-100,V-CAM 1,VCAM-1

表达区间及表达系统(Source)

Human VCAM-1, His Tag (VC1-H5224) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Pro 697 (Accession # NP_001069).

Predicted N-terminus: Phe 25

蛋白结构(Molecular Characterization)

Online(Phe 25 - Pro 697) NP_001069

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 74.9 kDa. The protein migrates as 80-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human VCAM-1, His Tag (Cat. No. VC1-H5224) SDS-PAGE gel

Human VCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human VCAM-1, His Tag (Cat. No. VC1-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 0.01-0.156 μg/mL (QC tested).

Protocol

背景(Background)

Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.

 

前沿进展

 
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VCAM-1靶点信息
英文全称:Vascular cell adhesion molecule 1
中文全称:血管细胞粘附分子1
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:1详情
最高研发阶段:批准上市
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