Human TIGIT, Fc Tag (TIT-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
Predicted N-terminus: Met 22
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 39.7 kDa. The protein migrates as 45-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human TIGIT, Fc Tag (Cat. No. TIT-H5254) was more than 90% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.
Immobilized Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.6-10 ng/mL (QC tested).
Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.313-10 μg/mL (Routinely tested).
Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).
Loaded Human TIGIT, Fc Tag (Cat. No. TIT-H5254) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 0.98 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS assay shows that recombinant Human TIGIT, Fc Tag (Cat. No. TIT-H5254) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 μg/mL (Routinely tested).
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.