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Human Syndecan-1 (L136Q) Protein, His Tag

分子别名(Synonym)

SDC1,Syndecan-1,CD138,SYND1,SDC

表达区间及表达系统(Source)

Human Syndecan-1 (L136Q), His Tag (SD1-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1(L136Q)).

Predicted N-terminus: Gln 23

蛋白结构(Molecular Characterization)

Online(Gln 23 - Gly 254) P18827-1 (L136Q)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.7 kDa. The protein migrates as 44 kDa and 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Syndecan-1 (L136Q), His Tag (Cat. No. SD1-H5228) SDS-PAGE gel

Human Syndecan-1 (L136Q), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Syndecan-1 (L136Q), His Tag (Cat. No. SD1-H5228) at 1 μg/mL (100 μL/well) can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.2-10 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human Syndecan-1 (L136Q), His Tag (Cat. No. SD1-H5228) with a linear range of 2-39 ng/mL (Routinely tested).

Protocol

背景(Background)

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

 

前沿进展

 
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Syndecan-1靶点信息
英文全称:Syndecan-1
中文全称:多配体蛋白聚糖-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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