分子别名(Synonym)
SCARB2,CD36L2,LIMP2,LIMPII
表达区间及表达系统(Source)
Human SCARB2, Fc Tag (SC2-H5250) is expressed from human 293 cells (HEK293). It contains AA Arg 27 - Thr 432 (Accession # AAH21892).
Predicted N-terminus: Arg 27
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 72.6 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human SCARB2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA
Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human SCARB2, Fc Tag (Cat. No. SC2-H5250) with a linear range of 0.02-1.25 μg/mL (Routinely tested).
Protocol
背景(Background)
Scavenger receptor class B member 2 (SCARB2) is also known as Lysosome membrane protein 2 (LIMP2), 85 kDa lysosomal membrane sialoglycoprotein (LGP85), CD36 antigen-like 2 (CD36L2, LIMP2), which belongs to the CD36 family. SCARB2 acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.