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 >  Protein>Neprilysin >MME-H521b

Human Neprilysin / MME / CD10 Protein, Tag Free (active enzyme)

分子别名(Synonym)

MME,CALLA,CD10,DKFZp686O16152,MGC126681,MGC126707,NEP,SFE,Neprilysin

表达区间及表达系统(Source)

Human Neprilysin, Tag Free (MME-H521b) is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2 ).

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蛋白结构(Molecular Characterization)

Neprilysin Structure

This protein carries no "tag"

The protein has a calculated MW of 80.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Neprilysin SDS-PAGE

Human Neprilysin, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)

Measured by its ability to cleave the fluorogenic peptide substrate, MCA-RPPGFSAFK(DNP)-OH. The specific activity is >1500 pmol/min/μg (QC tested).

Protocol

 

背景(Background)

Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110-kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).

 

前沿进展

 
 
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Neprilysin靶点信息
英文全称:Neprilysin
中文全称:脑啡肽酶
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:6详情
最高研发阶段:批准上市
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