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 >  Protein>IL-18BP >ILP-H5253

Human IL-18BP Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL18BP,IL18BPa,Tadekinig-alfa

表达区间及表达系统(Source)

Human IL-18BP, Fc Tag (ILP-H5253) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).

Predicted N-terminus: Thr 31

Request for sequence

蛋白结构(Molecular Characterization)

IL-18BP Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 44.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-18BP SDS-PAGE

Human IL-18BP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-18BP SEC-MALS

The purity of Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) is more than 85% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-SPR

IL-18BP SPR

Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.

 

前沿进展

 
 
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