Recombinant Proteins iso9001 ISO13485
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Human IL-18BP Protein, His Tag (MALS verified)

分子别名(Synonym)

IL18BP,IL18BPa,Tadekinig-alfa

表达区间及表达系统(Source)

Human IL-18BP, His Tag (ILP-H5222) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).

Predicted N-terminus: Thr 31

蛋白结构(Molecular Characterization)

Online(Thr 31 - Gly 194) NP_001138529

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.5 kDa. The protein migrates as 32-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-18BP, His Tag (Cat. No. ILP-H5222) SDS-PAGE gel

Human IL-18BP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human IL-18BP, His Tag (Cat. No. ILP-H5222) MALS images

The purity of Human IL-18BP, His Tag(Cat. No. ILP-H5222) was more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human IL-18BP, His Tag (Cat. No. ILP-H5222) inhibits the IL-18-induced IFN-gamma secretion by KG‑1 cells. The ED50 for this effect is 0.019-0.053 µg/mL in the presence of 30 ng/mL of Recombinant Human IL‑18 (Routinely tested).

Protocol

 

背景(Background)

Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.

 

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