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 >  Protein>IgG1 Fc >IG1-H5225

Human IgG1 Fc Protein, His Tag (MALS verified)

分子别名(Synonym)

IgG1

表达区间及表达系统(Source)

Human IgG Fc, His Tag (IG1-H5225) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1).

Predicted N-terminus: Glu 99

Request for sequence

蛋白结构(Molecular Characterization)

Online(Glu 99 - Lys 330) P01857-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.9 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IgG Fc, His Tag (Cat. No. IG1-H5225) SDS-PAGE gel

Human IgG Fc, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IgG Fc, His Tag (Cat. No. IG1-H5225) MALS images

The purity of Human IgG Fc, His Tag (Cat. No. IG1-H5225) is more than 95% and the molecular weight of this protein is around 50-66 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Human IgG Fc, His Tag (Cat. No. IG1-H5225) with an affinity constant of 1.60 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

 

 

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