Recombinant Proteins iso9001 ISO13485
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Human HGF R / c-MET Protein, Fc Tag (MALS verified)

分子别名(Synonym)

MET,AUTS9,HGFR,RCCP2,c-Met

表达区间及表达系统(Source)

Human HGF R, Fc Tag (MET-H5256) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).

Predicted N-terminus: Glu 25 (α chain) & Ser 308 (β chain Fc chimera)

蛋白结构(Molecular Characterization)

Online(Glu 25 - Thr 932) AAI30421.1

Human HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa (α chain) and 95.3 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and 106-116 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human HGF R, Fc Tag (Cat. No. MET-H5256) SDS-PAGE gel

Human HGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human HGF R, Fc Tag (Cat. No. MET-H5256) MALS images

The purity of Human HGF R, Fc Tag (Cat. No. MET-H5256) was more than 90% and the molecular weight of this protein is around 310-320 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human HGF, His Tag (Cat. No. HGF-H52H3) at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag (Cat. No. MET-H5256) with a linear range of 0.6-20 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human HGF R, Fc Tag (Cat. No. MET-H5256) at 5 μg/mL (100 μL/well) can bind Human HGF, His Tag (Cat. No. HGF-H52H3) with a linear range of 5-78 ng/mL (Routinely tested).

Protocol

背景(Background)

Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.

 

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HGF R靶点信息
英文全称:Hepatocyte growth factor receptor
中文全称:肝细胞生长因子受体
种类:Homo sapiens
上市药物数量:4详情
临床药物数量:46详情
最高研发阶段:批准上市
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