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 >  Protein>Growth Hormone R >GHR-H5222

Human Growth Hormone R (GHR) Protein, His Tag (MALS verified)

分子别名(Synonym)

GHR,GHBP,GH receptor

表达区间及表达系统(Source)

Human GHR, His Tag (GHR-H5222) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1 ).

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构(Molecular Characterization)

Growth Hormone R Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Growth Hormone R SDS-PAGE

Human GHR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Growth Hormone R SEC-MALS

The purity of Human GHR, His Tag (Cat. No. GHR-H5222) is more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Growth Hormone R ELISA

Immobilized Human GH, premium grade (Cat. No. GH1-H4118) at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag (Cat. No. GHR-H5222) with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

 

背景(Background)

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

文献引用(Citations)

 

前沿进展

 
 
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Growth Hormone R靶点信息
英文全称:Growth hormone receptor
中文全称:生长激素受体
种类:Homo sapiens
上市药物数量:28详情
临床药物数量:26详情
最高研发阶段:批准上市
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