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Biotinylated Human Fc gamma RIIA / CD32a (H167) Protein, Avitag™,His Tag (SPR & BLI & MALS verified)

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

表达区间及表达系统(Source)

Biotinylated Human CD32a (H167), Avitag,His Tag (CDA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1(H167)).

Predicted N-terminus: Ala 36

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 36 - Ile 218) P12318-1 (H167)

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) SDS-PAGE gel

Biotinylated Human CD32a (H167), Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) MALS images

The purity of Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) is more than 90% and the molecular weight of this protein is around 30-55 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) on SA Chip can bind Rituximab with an affinity constant of 0.74 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) on SA Biosensor, can bind Rituximab with an affinity constant of 0.59 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

 

前沿进展

 
 
Fc gamma RIIA / CD32a靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor II-a
中文全称:低亲和力免疫球蛋白γFc区受体II-a
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
前沿进展

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