登录 | 注册    关注公众号  
搜索

Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI & MALS verified)

分子别名(Synonym)

FCGR3A,CD16A,FCG3,FCGR3,IGFR3

表达区间及表达系统(Source)

Human CD16a (F176), His Tag (CDA-H5220) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).

Predicted N-terminus: Gly 17

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 17 - Gln 208) P08637-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.7 kDa. The protein migrates as 39-51 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD16a (F176), His Tag (Cat. No. CDA-H5220) SDS-PAGE gel

Human CD16a (F176), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CD16a (F176), His Tag (Cat. No. CDA-H5220) MALS images

The purity of Human CD16a (F176), His Tag (Cat. No. CDA-H5220) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Immobilized Human CD16a (F176), His Tag (Cat. No. CDA-H5220) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.70 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

Biotinylated Human SPR

Rituximab immobilized on CM5 Chip can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 1.82 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human CD16a (F176), His Tag (Cat. No. CDA-H5220) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 0.51 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

 

前沿进展

 
 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Fc gamma RIIIA / CD16a靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor III-A
中文全称:低亲和力免疫球蛋白γFc段受体III-A
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床二期
前沿进展

消息提示

请输入您的联系方式,再点击提交!

确定