登录 | 注册    关注公众号  
搜索
 >  生物标记素蛋白>EpCAM >EPM-H8254

Biotinylated Human EpCAM / TROP1, Fc Tag, ultra sensitivity (primary amine labeling)

热销产品推荐:

利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

EPCAM,TACSTD1,TROP1,CD326,DIAR5,EGP2,EGP314,EGP40,ESA,GA733-2,HNPCC8,HNPCC-8,KS1,4,KSA,M4S1,MIC18,MK1

表达区间及表达系统(Source)

MABSol® Biotinylated Human EpCAM / TROP1, Fc Tag (EPM-H8254) is expressed from human HEK293 cells. It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). It is the biotinylated form of Human EpCAM protein, Fc Tag (Cat # EPM-H5254).

Predicted N-terminus: Gln 24

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 24 - Lys 265) AAH14785.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 53.5 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human EpCAM, Fc Tag (Cat. No. EPM-H8254) SDS-PAGE gel

Biotinylated Human EpCAM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

背景(Background)

EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.

 

前沿进展

 
 
EpCAM靶点信息
英文全称:Epithelial cell adhesion molecule
中文全称:上皮细胞粘附分子
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:6详情
最高研发阶段:申请上市
前沿进展

消息提示

请输入您的联系方式,再点击提交!

确定