膜杰作
Star Staining
ViruStop
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
分子别名（Synonym）
Cadherin-1,CDH1,CD324,E-cadherin,Arc-1,ECAD,LCAM,UVO,E-CAD,Cadherin 1,CDH-1
表达区间及表达系统（Source）
Human E-Cadherin, Fc Tag (ECD-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 621 (Accession # AAI41839).
Predicted N-terminus: Gln 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 92.3 kDa. The protein migrates as 100 kDa and 120 kDa (Cadherin), and 48 kDa (Propeptide) under reducing (R) condition (SDS-PAGE) due to different glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human E-Cadherin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
文献引用（Citations）
PAK2 links cell survival to mechanotransduction and metabolism
Authors: Campbell HK, et al
Journal: J Cell Biol 2019
Application: Magnetic bead force assays & 3D atomic force microscopy
An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.
Authors: Eshghi A, et al.
Journal: Cell Microbiol 2018
Application: SPR
E-cadherin mediated lateral interactions between neighbor cells necessary for collective migration
Authors: Kevin Suffoletto, et al.
Journal: J Biomech 2018
Application:
背景（Background）
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).
