Recombinant Proteins iso9001 ISO13485
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Human DLL4 Protein, Fc Tag

分子别名(Synonym)

DLL4,Delta4

表达区间及表达系统(Source)

Human DLL4, Fc Tag (DL4-H5259) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).

Predicted N-terminus: Ser 27

蛋白结构(Molecular Characterization)

Online(Ser 27 - Pro 524) NP_061947.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 80.9 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human DLL4, Fc Tag (Cat. No. DL4-H5259) SDS-PAGE gel

Human DLL4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human DLL4, Fc Tag (Cat. No. DL4-H5259) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 1-16 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Mouse NOTCH1, His Tag (Cat. No. NO1-M52H8) at 2 μg/mL (100 μL/well) can bind Human DLL4, Fc Tag (Cat. No. DL4-H5259) with a linear range of 0.5-8 ng/mL (Routinely tested).

Protocol

背景(Background)

Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4). DLL4 contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 plays a role in the Notch signaling pathway. DLL4 activates Notch-1 and Notch-4.

 

前沿进展

 
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DLL4靶点信息
英文全称:Delta-like protein 4
中文全称:δ样蛋白-4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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