Human DLL4, Fc Tag (DL4-H5259) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).
Predicted N-terminus: Ser 27
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.9 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human DLL4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human DLL4, Fc Tag (Cat. No. DL4-H5259) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 1-16 ng/mL (QC tested).
Immobilized Mouse NOTCH1, His Tag (Cat. No. NO1-M52H8) at 2 μg/mL (100 μL/well) can bind Human DLL4, Fc Tag (Cat. No. DL4-H5259) with a linear range of 0.5-8 ng/mL (Routinely tested).
Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4). DLL4 contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 plays a role in the Notch signaling pathway. DLL4 activates Notch-1 and Notch-4.