Human CD47, His Tag (CD7-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
Predicted N-terminus: Gln 19
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.6 kDa. The protein migrates as 33-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Human CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD47, His Tag (Cat. No. CD7-H5227) is more than 90% and the molecular weight of this protein is around 24-36 kDa verified by SEC-MALS.
Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 31-125 ng/mL (QC tested).
Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 0.4-3 ng/mL (Routinely tested).
Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-Human CD47 MAb (Human IgG4) on AHC Biosensor, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 6.18 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: D Liu, W Huang, B Chen, J Liu
Journal: US20200377593A1 2020
Application: Flow cytometry
Authors: Wang Y, Ni H, Zhou S, et al.
Journal: Cancer Immunol Immunother 2020
Authors: PT Manning, et al
Journal: US20190309066A1 2019
Authors: Manning Pamela T, et al.
Journal: US20180142019A1 2018
Authors: Yu XY, et al.
Journal: Biochimie 2018
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.