Human CD44, Fc Tag (HPLC-verified) (PG1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Pro 220 (Accession # AAH04372).
Predicted N-terminus: Gln 21
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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.7 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD44, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) was greater than 90% as determined by SEC-HPLC.
Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) with a linear range of 0.156-2.5 μg/mL (Routinely tested).
Immobilized Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 10-156 ng/mL (Routinely tested).
Authors: Bhattacharya D, et al.
Journal: J MATER CHEM B 2017
Application: Fluorescence polarization assay
CD44 antigen is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion and migration. CD44 is expressed in a large number of mammalian cell types. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 function is controlled by its posttranslational modifications. One critical modification involves discrete sialofucosylations rendering the selectin-binding glycoform of CD44 called HCELL (for Hematopoietic Cell E-selectin/L-selectin Ligand). CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms.