Recombinant Proteins iso9001 ISO13485
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Human CD40 / TNFRSF5 Protein, His Tag (MALS verified)

分子别名(Synonym)

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

表达区间及表达系统(Source)

Human CD40, His Tag (MALS verified) (CD0-H5228) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).

Predicted N-terminus: Glu 21

蛋白结构(Molecular Characterization)

Online(Glu 21 - Arg 193) P25942-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.1 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD40, His Tag (MALS verified) (Cat. No. CD0-H5228) SDS-PAGE gel

Human CD40, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CD40, His Tag (MALS verified) (Cat. No. CD0-H5228) MALS images

The purity of Human CD40, His Tag (MALS verified) (Cat. No.CD0-H5228) was more than 95% and the molecular weight of this protein is around 23-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD40, His Tag (Cat. No. CD0-H5228) at 5 μg/mL (100 μL/well) can bind Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5267) with a linear range of 0.5-8 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

文献引用(Citations)

背景(Background)

Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).

 

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CD40靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 5
中文全称:肿瘤坏死因子受体超家族成员5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:17详情
最高研发阶段:临床二期
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