Recombinant Proteins iso9001 ISO13485
搜索
 >  其他蛋白>CD38 >CD8-H5224

Human CD38 Protein, His Tag (MALS verified)

分子别名(Synonym)

CD38,T10,cADPr hydrolase 1

表达区间及表达系统(Source)

Human CD38, His Tag (CD8-H5224) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766).

Predicted N-terminus: Val 43

蛋白结构(Molecular Characterization)

Online(Val 43 - Ile 300) NP_001766

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.7 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM MES, 100 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD38, His Tag (Cat. No. CD8-H5224) SDS-PAGE gel

Human CD38, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CD38, His Tag (Cat. No. CD8-H5224) MALS images

The purity of Human CD38, His Tag (Cat. No.CD8-H5224) was more than 95% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD38, His Tag (Cat. No. CD8-H5224) at 5 μg/mL (100 μL/well) can bind Anti-CD38 MAb, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-CD38 mAb (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CD38, His Tag (Cat. No. CD8-H5224) with an affinity constant of 4.98 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.

 

前沿进展

 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
CD38靶点信息
英文全称:Lymphocyte differentiation antigen CD38
中文全称:淋巴细胞分化抗原CD38
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:14详情
最高研发阶段:批准上市
查看更多信息
推荐会议
查看全部会议

消息提示

请输入您的联系方式,再点击提交!

确定