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 >  Cell>NF-κB >SCJUR-STF113

NF-kB (Luc) Jurkat Reporter Cell Development Service

For research use only.

描述(Description)

The NF-kB (Luc) Jurkat Reporter Cell was engineered with the NF-kB response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NF-kB-RE mediated luminescence.

应用说明(Application)

• The discovery of activators or inhibitors by the NF-kB signaling bioactivity

• Transfection host for some receptors concerning the NF-kB signaling pathway

NF-κB Assay Principles

生长特性(Growth Properties)

Suspension

筛选标记(Selection Marker)

NA

培养基(Culture Medium)

RPMI-1640 + 10% FBS

冻存液(Freeze Medium)

Serum-free cell cryopreservation medium

装量(Quantity)

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储(Storage)

Frozen in liquid nitrogen.

支原体检测(Mycoplasma Testing)

Negative

无菌检测(Sterility Testing)

Negative

使用说明(Instructions for Use)

See data sheet for detailed culturing and assay protocol.

 

Signaling Bioassay

NF-κB SIGNALING

Response to human TNFα protein (RLU).
The NF-kB (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TNFα protein (AcroBiosystems, Cat.No.TNA-H4211). The EC50 was approximately 0.01751 μg/mL.

Protocol

NF-κB SIGNALING

Response to human TNFα protein (FOLD).
The NF-kB (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TNFα protein (AcroBiosystems, Cat.No.TNA-H4211). The max induction fold was approximately 106.

Protocol

 

Passage Stability

NF-κB PASSAGE

Passage stability analysis by Signaling Bioassay.
The continuously growing NF-kB (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TNFα protein (AcroBiosystems, Cat.No.TNA-H4211). Human TNFα protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 11-21.

Protocol

 

背景(Background)

The nuclear factor-κB (NF-κB) transcription factor family, which has been considered the central mediator of the inflammatory process and a key participant in innate and adaptive immune responses, could accelerate cell proliferation, inhibit apoptosis, promote cell migration and invasion, and stimulate angiogenesis and metastasis. Notably, in many types of cancer, both in malignant cells and in the tumour microenvironment, NF­κB is constitutively active. Most of the increased NF-κB activity observed in solid malignancies is due to elevated production of IKK activating cytokines, including tumour necrosis factor (TNF) and IL-1.

 

License Disclosure

This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.

 

前沿进展

 
 
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order.cn@acrobiosystems.com
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NF-κB靶点信息
英文全称:Nuclear factor NF-kappa-B complex
中文全称:核因子NF-κB复合体
种类:Homo sapiens
上市药物数量:11详情
临床药物数量:38详情
最高研发阶段:批准上市
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