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 >  Protein>Cadherin-6 >CA6-H82E3

Biotinylated Human Cadherin-6 / KCAD / CDH6 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

CDH6,CAD6,KCAD,K-cadherin,Cadherin-6

表达区间及表达系统(Source)

Biotinylated Human Cadherin-6 Protein, His,Avitag (CA6-H82E3) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Ala 615 (Accession # P55285-1 ).

Predicted N-terminus: Thr 19

Request for sequence

蛋白结构(Molecular Characterization)

Cadherin-6 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 70.0 kDa. The protein migrates as 53 kDa, 65 kDa and 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cadherin-6 SDS-PAGE

Biotinylated Human Cadherin-6 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Cadherin-6 SEC-MALS

The purity of Biotinylated Human Cadherin-6 Protein, His,Avitag (Cat. No. CA6-H82E3) is more than 90% and the molecular weight of this protein is around 75-100 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Cadherin-6 ELISA

Immobilized Human Anti-Cadherin-6 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Cadherin-6 Protein, His,Avitag (Cat. No. CA6-H82E3) with a linear range of 0.2-16 ng/mL (QC tested).

Protocol

Cadherin-6 ELISA

Immobilized Biotinylated Human Cadherin-6 Protein, His,Avitag (Cat. No. CA6-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Anti-Cadherin-6 with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Cadherin-6 (CDH6) is also known as Kidney cadherin (K-cadherin or KCAD), is a a type II classical cadherin from the cadherin superfamily. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH6 / KCAD contains five cadherin domains. CDH6 is highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression and also expressed in certain liver and kidney carcinomas.

 

前沿进展

 
 
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Cadherin-6靶点信息
英文全称:Cadherin-6
中文全称:钙粘蛋白6
种类:
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床三期
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