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Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (CDG-H52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1(CD3E) & P09693-1(CD3G)).

Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

蛋白结构(Molecular Characterization)

Online(Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)) P07766-1 (CD3E) & P09693-1 (CD3G)

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (Cat. No. CDG-H52W9) SDS-PAGE gel

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (Cat. No. CDG-H52W9) MALS images

The purity of Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) was more than 90% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 2 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with a linear range of 0.4-6 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a)) with an affinity constant of 39.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Human CD3 antibody (UCHT1) with an affinity constant of 1.59 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Bispecific T-cell Engager (CD3 X BCMA) with an affinity constant of 1.13 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

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