膜杰作
Star Staining
描述（Description）
HEK293/Human Transferrin R Stable Cell Line
应用说明（Application）
Binding assay by FACS and cell based ELISA.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin B (100μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
10% DMSO + 90% FBS
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
活性（Bioactivity）
Fig. FACS analysis of Transferrin R on HEK293/Human Transferrin R Stable Cell Line.
FACS assay shows that Anti-Transferrin Receptor antibody can bind to HEK293/Human Transferrin R Stable Cell Line. HEK293/Human Transferrin R Stable Cell Line was red line, Negative control HEK293 cells was grey line (QC tested).
Protocol
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背景（Background）
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
