膜杰作
Star Staining
分子别名（Synonym）
IFNAR1,IFNAR,CRF2-1,IFN-R-1,IFNAR-1
表达区间及表达系统（Source）
Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (IF1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1).
Predicted N-terminus: Lys 28
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 50 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (Cat. No. IF1-H82E3) is more than 90% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (Cat. No. IF1-H82E3) captured on Biotin CAP-Series S Sensor Chip can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 0.177 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
Interferon alpha/beta receptor 1 ( IFNAR1) is also known as Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1 (CRF2-1), Type I interferon receptor 1, IFNAR, which belongs to the type II cytokine receptor family. IFNAR1 /IFNAR contains four fibronectin type-III domains. IFNAR1 associates with IFNAR2 to form the type I interferon receptor. IFNAR1 is receptor for interferons alpha and beta. IFNAR1 can transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway.
