分子别名(Synonym)
OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
表达区间及表达系统(Source)
Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (OXL-H82F5) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).
Predicted N-terminus: Gly
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
蛋白结构(Molecular Characterization)
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 75.7 kDa. The protein migrates as >145 kDa under reducing (R) condition, and 135-235 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 0.01 EU per μg by the LAL method.
宿主蛋白残留(Host Cell Protein)
<0.5 ng/μg of protein tested by ELISA.
宿主核酸残留(Host Cell DNA)
<0.02 ng/μg of protein tested by qPCR.
无菌(Sterility)
The sterility testing was performed by membrane filtration method.
支原体(Mycoplasma)
Negative.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, PH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (Cat. No. OXL-H82F5) is more than 90% and the molecular weight of this protein is around 175-205 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 1 μg/mL (100 μL/well) can bind Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (Cat. No. OXL-H82F5) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Immobilized Human Olfactory Marker Protein, His Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human OX40 Ligand Protein, Avitag,Fc Tag, premium grade (Cat. No. OXL-H82F5) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
背景(Background)
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.