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 >  Protein>HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) >HLD-H52H4

Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Monomer, MALS verified)

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分子别名(Synonym)

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK)

表达区间及表达系统(Source)

Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (HLD-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVGADGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVGADGVGK).

Predicted N-terminus: Gly 25 & Ile 21

Request for sequence

蛋白结构(Molecular Characterization)

Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12D peptide.

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 41-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) SDS-PAGE

Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) SEC-MALS

The purity of Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) ELISA

Immobilized Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) ELISA

Immobilized Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) SPR

Anti-HLA class I Antibody, Human IgG1 (W6/32) captured on Protein A Chip can bind Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) with an affinity constant of 1.06 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Biotinylated Human HLA-A*1101 KRASG12D (VVGADGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVGADGVGK peptide of the KRASG12D.

 

前沿进展

 
 
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