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 >  Protein>IL-2 R beta & IL-2 R alpha & IL-2 R gamma >ILG-C52W3

Cynomolgus IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, His Tag&Twin-Strep Tag (MALS verified)

分子别名(Synonym)

IL-2 R beta & IL-2 R alpha & IL-2 R gamma

表达区间及表达系统(Source)

Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag (ILG-C52W3) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Glu 213 & Leu 23 - Pro 255 (Accession # Q38J85-1 & H6WS54 & Q38JL2-1 ).

Predicted N-terminus: Glu 22 & Leu 23

Request for sequence

蛋白结构(Molecular Characterization)

Cynomolgus IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 30.1 kDa (IL-2RB) and 55.9 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 37-44 kDa and 85-100 kDa respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-2 R beta & IL-2 R alpha & IL-2 R gamma SDS-PAGE

Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-2 R beta & IL-2 R alpha & IL-2 R gamma SEC-MALS

The purity of Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-C52W3) is more than 85% and the molecular weight of this protein is around 120-140 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-2 R beta & IL-2 R alpha & IL-2 R gamma ELISA

Immobilized Cynomolgus IL-2, His Tag (Cat. No. IL2-C5249) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-C52W3) with a linear range of 2-78 ng/mL (QC tested).

Protocol

IL-2 R beta & IL-2 R alpha & IL-2 R gamma ELISA

Immobilized Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-C52W3) at 5 μg/mL (100 μL/well) can bind Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

IL-2 R beta & IL-2 R alpha & IL-2 R gamma SPR

Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-C52W3) captured on CM5 chip via anti-His antibody can bind Human IL-2, Tag Free with an affinity constant of 29.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-2 R beta & IL-2 R alpha & IL-2 R gamma BLI

Loaded Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) on Protein A Biosensor, can bind Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-C52W3) with an affinity constant of 0.907 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

 

前沿进展

 
 
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IL-2 R beta & IL-2 R alpha & IL-2 R gamma靶点信息
英文全称:Interleukin-2
中文全称:白细胞介素-2
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:31详情
最高研发阶段:批准上市
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