膜杰作
Star Staining
分子别名（Synonym）
IFNW1,IFN-omega
表达区间及表达系统（Source）
Human IFN-omega Protein, His Tag (IFA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 24-Ser 195 (Accession # P05000).
Predicted N-terminus: Cys 24
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 21.8 kDa. The protein migrates as 27-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IFN-omega Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IFN-omega Protein, His Tag (Cat. No. IFA-H52H3) is more than 90% and the molecular weight of this protein is around 18-30 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Human IFN-omega Protein, His Tag (Cat. No. IFA-H52H3) on NTA Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. IF1-H5253) with an affinity constant of 245 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
背景（Background）
The protein encoded by this gene is an interferon and possesses antiviral activity. The encoded protein binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. This intronless gene has several pseudogenes spread throughout the genome.
