膜杰作
Star Staining
分子别名（Synonym）
A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R, IGFBP3R1, KPA, LRP, LRP1A, TGFBR5
表达区间及表达系统（Source）
Human cluster Ⅲ Protein, Fc Tag (LR1-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 2522 - Ile 2941 (Accession # Q07954-1).
Predicted N-terminus: Ser 2522
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 72.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human cluster Ⅲ Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human cluster Ⅲ Protein, Fc Tag (Cat. No. LR1-H5255) is more than 90% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Unconjugated Human PDGF-BB, His,Avitag (Cat. No. PDB-H5127) on NTA Biosensor, can bind Human cluster Ⅲ Protein, Fc Tag (Cat. No. LR1-H5255) with an affinity constant of 20 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human cluster Ⅲ Protein, Fc Tag (Cat. No. LR1-H5255) on Protein A Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 8.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Low density lipoprotein receptor-related protein 1 (LRP1), also known as alpha-2-macroglobulin receptor (A2MR), apolipoprotein E receptor (APOER) or cluster of differentiation 91 (CD91), is a protein forming a receptor found in the plasma membrane of cells. The encoded preproprotein is proteolytically processed by furin to generate 515 kDa and 85 kDa subunits that form the mature receptor. LRP1 is also a key signalling protein and, thus, involved in various biological processes, such as lipoprotein metabolism and cell motility, and diseases, such as neurodegenerative diseases, atherosclerosis, and cancer.
