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Human Anti-SARS-CoV-2 (BF.7) Antibody IgA1 Titer Serologic Assay Kit (Spike RBD)

For research use only.

IDComponentsSize
RAS153-C01Pre-coated SARS-CoV-2 Spike RBD (BF.7) Microplate1 plate
RAS153-C02Anti-SARS-CoV-2 Antibody (Control, IgA1)100 μL
RAS153-C03HRP-Anti-Human IgA130 μL
RAS153-C0410xWashing Buffer 50 mL
RAS153-C05Dilution Buffer50 mL
RAS153-C06Substrate Solution12 mL
RAS153-C07Stop Solution7 mL

背景(Background)

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

应用说明(Application)

The kit is developed for titer measurement of Anti-SARS-CoV-2 (BF.7) IgA1 antibody (Spike RBD) in human serum.

It is for research use only.

存储(Storage)

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理(Assay Principles)

This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgA1 by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD(BF.7). First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Anti-Human IgA1 to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm. The OD Value reflects the amount of antibody bound.

Your experiment will include 4 simple steps:

a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

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