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 >  Protein>PAR1 >PA1-H52P6

Human PAR1 Full Length Protein (VLP)

分子别名(Synonym)

F2R,coagulation factor II thrombin receptor,CF2R, HTR, PAR-1, PAR1, TR

表达区间及表达系统(Source)

Human PAR1 Full Length Protein-VLP (PA1-H52P6) is expressed from human 293 cells (HEK293). It contains AA Ser 42 - Thr 425 (Accession # P25116-1).

Predicted N-terminus: Asp

蛋白结构(Molecular Characterization)

The protein has a calculated MW of 91.2 kDa | 56.1 kDa.

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
 

活性(Bioactivity)-ELISA

PAR1 ELISA

Immobilized Human PAR1 Full Length Protein-VLP (Cat. No. PA1-H52P6) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human PAR1 antibody, Mouse IgG1 with a linear range of 0.1-1.3 μg/mL (QC tested).

Protocol

 

均一性(Identity)-DLS

PAR1 DLS

The mean peak Radius of VLP is 50-70 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

 

背景(Background)

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants.

 

前沿进展

 
 
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PAR1靶点信息
英文全称:Protease-activated receptor-1
中文全称:蛋白酶活化受体1
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上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
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