抗体来源(Source)
HRP conjugated Monoclonal Anti-MMAE&MMAF Antibody,Mouse IgG1 is recombinantly produced from human 293 cells (HEK293).
亚型(Isotype)
Mouse IgG1/kappa
特异性(Specificity)
Specifically recognizes the target-MMAE.
应用(Application)
This Monoclonal anti-MMAE antibody is designed for use in ELISA and PK environment.
纯度(Purity)
>95% as determined by SDS-PAGE.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
HRP conjugated Monoclonal Anti-MMAE&MMAF Antibody,Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)-ELISA
Immobilized Disitamab Vedotin at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-MMAE&MMAF Antibody,Mouse IgG1 (Cat. No. MME-PLS104) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
背景(Background)
Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several different cancer types. Anti-MMAE antibody is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: PK, PD, Immunoassay and ELISA.