膜杰作
Star Staining
分子别名（Synonym）
LYPD1,Ly6/PLAUR domain-containing protein 1,FLJ41033,LY6/PLAUR domain containing 1,LYPDC1,PHTS,Putative HeLa tumor suppressor
表达区间及表达系统（Source）
Human LYPD1 Protein, Mouse IgG2a Fc Tag (LY1-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Gly 115 (Accession # Q8N2G4-1).
Predicted N-terminus: Leu 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the C-terminus
The protein has a calculated MW of 37.3 kDa. The protein migrates as 43-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human LYPD1 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human LYPD1 Protein, Mouse IgG2a Fc Tag (Cat. No. LY1-H5258) is more than 90% and the molecular weight of this protein is around 155-175 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human LYPD1 Protein, Mouse IgG2a Fc Tag (Cat. No. LY1-H5258) at 1 μg/mL (100 μL/well) can bind Anti-LYPD1 antibody with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
背景（Background）
Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro increases receptor desensitization and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. May play a role in the intracellular trafficking of alpha-4:beta-2 and alpha-7-containing nAChRs and may inhibit their expression at the cell surface. May be involved in the control of anxiety.
