分子别名(Synonym)
CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90
表达区间及表达系统(Source)
Biotinylated Rat Transferrin R Protein, His,Avitag (TFR-R82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 87 - Phe 761 (Accession # Q99376-1).
Predicted N-terminus: Gly
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 79.8 kDa. The protein migrates as 70-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Biotinylated Rat Transferrin R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA
Immobilized Biotinylated Rat Transferrin R Protein, His,Avitag (Cat. No. TFR-R82E3)at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Transferrin Protein, Mouse IgG2a Fc Tag (Cat. No. TRN-M525b) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Mouse Transferrin Protein, Mouse IgG2a Fc Tag (Cat. No. TRN-M525b) at 2 μg/mL (100 μL/well) can bind Biotinylated Rat Transferrin R Protein, His,Avitag (Cat. No. TFR-R82E3) with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
背景(Background)
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.