膜杰作
Star Staining
描述（Description）
The Human IL-11 R alpha (Luc) HEK293 Reporter Cell was engineered to express STAT3 signaling response element. When stimulated with human IL-11 protein, the IL-11/ IL-11 R alpha interaction drives STAT3-mediated luminescence. Inhibition of IL-11 binding to IL-11 R alpha by either anti-IL-11 or anti-IL-11 R alpha antibodies results in a decrease in luminescence.
应用说明（Application）
• Screen for anti-human IL-11 or anti-human IL-11 R alpha neutralizing antibody.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin (40 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Application
Inhibition of human IL-11 protein-induced reporter activity by anti-human IL-11 neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-11 protein (Cat.No.IL1-H5243) with a final concentration of 0.2 μg/mL. The EC50 of anti-human IL-11 neutralizing antibody is approximately 1.047 μg/mL.
Protocol
Signaling Bioassay
Response to human IL-11 protein (RLU).
The Human IL-11 R alpha (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-11 protein (Cat.No.IL1-H5243). The EC50 was approximately 0.12 μg/mL.
Protocol
Response to human IL-11 protein (FOLD).
The Human IL-11 R alpha (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-11 protein (Cat.No.IL1-H5243). The max induction fold was approximately 31.
Protocol
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背景（Background）
IL-11 and IL-6 are signal transducer and activator of transcription 3 (STAT3) activators and frequently found upregulated in colorectal cancer (CRC). STAT3 activation in tumor epithelial cells is critically involved in the pathogenesis of CRC. Cancer-associated fibroblasts (CAFs) can modulate the tumor microenvironment in CRC. The latest research indicated that phosphorylation of STAT3 (pSTAT3) expression in CAFs is inversely correlated with the survival in CRC patients. IL-6 and IL-11 induce partly overlapping gene expression patterns in fibroblast subsets in CRC. In a summary, STAT3 activation in CAFs plays a key role in the cross-talk to the tumor epithelium.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
