膜杰作
Star Staining
分子别名（Synonym）
Apolipoprotein E,APOE,Apo-E
表达区间及表达系统（Source）
Mouse Apolipoprotein E Protein, His Tag (APE-M52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Gln 311 (Accession # P08226).
Predicted N-terminus: Glu 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 35.9 kDa. The protein migrates as 14 kDa and 35 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse Apolipoprotein E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human LDL R, Fc Tag (Cat. No. LDR-H5254) at 5 μg/mL (100 μL/well) can bind Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) with a linear range of 0.02-2.5 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) immobilized on CM5 Chip can bind Mouse LDL R, His Tag (Cat. No. LDR-M52H8) with an affinity constant of 73.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.
