膜杰作
Star Staining
分子别名（Synonym）
BXMAS1, CD307, CD307e, FCRH5, IRTA2, PRO820
表达区间及表达系统（Source）
Biotinylated Human FCRL5, His,Avitag (FC5-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Gly 851 (Accession # Q96RD9-1).
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 95.0 kDa. The protein migrates as 105-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human FCRL5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human FCRL5, His,Avitag (Cat. No. FC5-H82E3) is more than 85% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Biotinylated Human FCRL5 Protein, His,Avitag (Cat. No. FC5-H82E3) capture on NTA-Series S sensor chip can bind Ipilimumab with an affinity constant of 18.5 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells.
