膜杰作
Star Staining
产品描述（Product Details）
This product is a purified recombinant inorganic pyrophosphatase (PPase) in Escherichia coli.
Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
P2O7-4+H2O+PPase→2HPO4-2
PPase can hydrolyze inorganic pyrophosphate generated with the reaction to avoid its inhibition of the reaction system. It can be used to increase RNA yield in reverse transcription reactions.
This enzyme retains 80% activity after incubation at 100°C for 4 hours.
应用说明（Application）
- Increase RNA yield in transcription reaction;
- Enhance DNA replication;
- Hydrolyze inorganic pyrophosphate.
单位定义（Unit Definition）
One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (at 75°C in 50 mM Tricine, pH 8.5, 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml for 10 minute).
质量控制（Quility Control）
No endodeoxyribonucleases, exodeoxyribonucleases and Ribonucleases residues.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris,100 mM NaCl,1 mM DTT,0.1 mM EDTA,pH 8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable for up to 12 months at -20℃ from date of receipt.
注意事项（Notes）
This product is active in a variety of reaction buffers and can usually be added directly to amplification experiments such as HDA and LAMP.
