膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | IL-8 |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | <5.0 pg/mL |
| Standard Curve Range | 6.25 pg/mL-800 pg/mL |
| Assay Time | 2 hr |
| Suitable Sample Type | For the quantitative determination of human IL-8 in serum, plasma and cell supernatant. |
| Sample volume | 50 μL |
组分（Materials Provided）
| ID | Components | Size |
| CRB004-C01 | Pre-coated Anti-IL-8 Antibody Microplate | 1 plate |
| CRB004-C02 | Human IL-8 Standard | 20 μg×2 |
| CRB004-C03 | Biotin-Anti-IL-8 Antibody Con. Solution | 100 μL |
| CRB004-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB004-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CRB004-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB004-C07 | 20× Washing Buffer | 50 mL |
| CRB004-C08 | 1× Dilution Buffer | 15 mL×2 |
| CRB004-C09 | Substrate Solution | 12 mL |
| CRB004-C10 | Stop Solution | 6 mL |
背景（Background）
ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-8 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
应用说明（Application）
The kit is developed for quantitative detection of IL-8 in human serum and cell culture supernates.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit is used to measure the concentrations of human Interleukin 8 (IL-8) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-8 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-8 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B004 is less than 5.0 pg/mL.
背景（Background）：IL-8
Interleukin 8, IL-8
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells during the inflammatory process. Like all chemokines, IL-8 is a small, soluble peptide (8–10 kDa).Interleukin-8 is synthesized primarily as a 99 amino acid peptide that is processed through signal sequence cleavage and N-terminal proteolysis to yield several active IL-8 isoforms , with peptides of 72 and 77 amino acids being most biologically relevant. The 72 amino acid peptide is the major form secreted by monocytes and macrophages in culture, whereas the 77 amino acid variant is the most abundant secretory product of nonimmune cells.
