登录 | 注册    关注公众号  
微信公众号
搜索
 >  Kits>IL-10 >CRS-B005

ClinMax™ Human IL-10 ELISA Kit

For research use only.

产品描述(Product Details)

Assay TypeSandwich-ELISA
AnalyteIL-10
Format96T
ReactivityHuman
Regulatory StatusRUO
Sensitivity<2.0 pg/mL
Standard Curve Range4.11 pg/mL-1000 pg/mL
Assay Time2 hr
Suitable Sample TypeFor the quantitative determination of human IL-10 in serum, plasma and cell supernatant.
Sample volume50 μL

组分(Materials Provided)

IDComponentsSize
CRB005-C01Pre-coated Anti-IL-10 Antibody Microplate1 plate
CRB005-C02Human IL-10 Standard60 μg×2
CRB005-C03Biotin-Anti-IL-10 Antibody Con. Solution100 μL
CRB005-C04Biotin-Antibody Dilution Buffer8 mL
CRB005-C05Streptavidin-HRP Con. Solution500 μL
CRB005-C06Streptavidin-HRP Dilution Buffer15 mL
CRB005-C0720× Washing Buffer50 mL
CRB005-C081× Dilution Buffer15 mL×2
CRB005-C09Substrate Solution12 mL
CRB005-C10Stop Solution6 mL

背景(Background)

ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-10 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

应用说明(Application)

The kit is developed for quantitative detection of IL-10 in human serum and cell culture supernates.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储(Storage)

Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理(Assay Principles)

This assay kit is used to measure the concentrations of human Interleukin 10 (IL-10) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-10 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-10 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.

 

典型数据-Typical Data Please refer to DS document for The assay protocol.

IL-10 TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B005 is less than 2.0 pg/mL.

 

背景(Background):IL-10

Interleukin 10, IL-10
IL-10 is a highly potent cytokine produced by a variety of cells. It regulates cell growth and differentiation, participates in inflammatory reactions, and immune responses, and is currently recognized as inflammation and immunosuppressive factor. It plays an important role in autoimmune diseases and inflammatory diseases.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-10靶点信息
英文全称:Interleukin-10
中文全称:白细胞介素-10
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细
相关产品
View All IL-10

消息提示

请输入您的联系方式,再点击提交!

确定