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 >  Protein>OX40 Ligand >OXL-C5243

Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag, active trimer (MALS verified)

分子别名(Synonym)

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统(Source)

Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag (OXL-C5243) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # A0A1D5QQH7 ).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 49.3 kDa. The protein migrates as 65-80 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

OX40 Ligand SDS-PAGE

Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

OX40 Ligand SEC-MALS

The purity of Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag (Cat. No. OXL-C5243) is more than 95% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

OX40 Ligand ELISA

Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag (Cat. No. OXL-C5243) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque OX40, Fc Tag (Cat. No. OX0-C5251) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

OX40 Ligand ELISA

Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag (Cat. No. OXL-C5243) at 2 μg/mL (100 μL/well) can bind Human OX40, Fc Tag (Cat. No. OX0-H5255) with a linear range of 0.1-1 ng/mL (Routinely tested).

Protocol

OX40 Ligand ELISA

Immobilized Cynomolgus / Rhesus macaque OX40, Fc Tag (Cat. No. OX0-C5251) at 5 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque OX40 Ligand, His Tag (Cat. No. OXL-C5243) with a linear range of 20-78 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

 

前沿进展

 
 
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OX40 Ligand靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 4
中文全称:OX40配体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:6详情
最高研发阶段:临床三期
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