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Anti-Human IgG Antibody-Acridinium ester (MALS verified)

抗体来源(Source)

Anti-Human IgG Antibody-Acridinium ester antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with IgG.

亚型(Isotype)

Mouse IgG1/kappa

特异性(Specificity)

This product is a specific antibody specifically reacts with IgG.

标记(Labeling)

Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.

蛋白标记度(Protein Ratio)

Passed as determined by binding MPCLIA.

应用(Application)

MPCLIA

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH6.3.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IgG SDS-PAGE

Anti-Human IgG Antibody-Acridinium ester on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IgG SEC-MALS

The purity of Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69) is more than 90% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-MPCLIA

IgG MPCLIA

Immobilized 0.04 μg /Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

Protocol

IgG MPCLIA

The MPCLIA assay shows that Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69) is stable at 37°C for 48 hours.

IgG MPCLIA

The MPCLIA assay shows that Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69) is stable after freezing and thawing 3 times.

 

背景(Background)

The Anti-Human IgG-Acridine Ester is an acridinium ester labeled Monoclonal mouse anti-human IgG antibody can be used to capture the human IgG in Chemiluminescence procedures.
The antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, cynomolgus and bovine serum proteins, but it is not sure if the product cross-react with immunoglobulins from other species.
Acridinium ester is one of the most commonly used excited substrates for protein labeling and widely used in automated instruments because of its high sensitivity with detection limits in the attomole range. Acridinium ester can be triggering by chemiluminescent substrate solution and generate intense signal, the result signals (relative light units, RLU) can be measured at 430 nm.
The Anti-human IgG-Acridine Ester is easy to capture the human IgG, and the bounded human IgG have no activity lost, this ready to use products could greatly save your protein labeling time and hassle, and help us get the best performance and highly reproducible results.

 

前沿进展

 
 
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IgG靶点信息
英文全称:Immunoglobulin G
中文全称:免疫球蛋白G
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
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