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 >  Protein>MAdCAM-1 >MAM-H52H4

Human MAdCAM-1 Protein, His Tag (MALS & SPR verified)

分子别名(Synonym)

MAdCAM-1,MAdCAM1,Mucosal vascular addressin cell adhesion molecule 1 transcript variant 1

表达区间及表达系统(Source)

Human MAdCAM-1, His Tag (MAM-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Gln 317 (Accession # Q13477-1).

Predicted N-terminus: Gln 19

Request for sequence

蛋白结构(Molecular Characterization)

MAdCAM-1 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 33.3 kDa. The protein migrates as 46-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

MAdCAM-1 SDS-PAGE

Human MAdCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

MAdCAM-1 SEC-MALS

The purity of Human MAdCAM-1, His Tag (Cat. No. MAM-H52H4) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-SPR

MAdCAM-1 SPR

Human MAdCAM-1, His Tag (Cat. No. MAM-H52H4) immobilized on CM5 Chip can bind Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) with an affinity constant of 0.227 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

 

背景(Background)

The mucosal addressin cell adhesion molecule-1 (MAdCAM-1) takes a key role in the endothelial adhesion and migration of lymphocytes to sites of inflammation in inflammatory disease. In vitro and in vivo data indicate that blockade of the adhesion molecule mucosal address in cell adhesion molecule (MAdCAM) pathway decreases leukocyte homing to the gut by inhibiting the interaction between MAdCAM and its ligand the α4β7 integrin expressed on lymphocytes.

 

前沿进展

 
 
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MAdCAM-1靶点信息
英文全称:Mucosal addressin cell adhesion molecule-1
中文全称:黏膜地址素细胞黏附分子-1
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
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