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 >  Protein>EphA5 >EP5-H52H5

Human EphA5 Protein, His Tag (MALS & SPR verified)

分子别名(Synonym)

Ephrin type-A receptor 5

表达区间及表达系统(Source)

Human EphA5, His Tag (EP5-H52H5) is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Pro 573 (Accession # P54756-1).

Predicted N-terminus: Pro 25

Request for sequence

蛋白结构(Molecular Characterization)

EphA5 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 62.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

EphA5 SDS-PAGE

Human EphA5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

EphA5 SEC-MALS

The purity of Human EphA5, His Tag (Cat. No. EP5-H52H5) is more than 95% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-SPR

EphA5 SPR

Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human EphA5, His Tag (Cat. No. EP5-H52H5) with an affinity constant of 0.576 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

背景(Background)

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EF1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EF5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance.

 

 

前沿进展

 
 
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EphA5靶点信息
英文全称:EPH Receptor A5
中文全称:A型肝配蛋白受体-5
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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