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 >  Cell>Fc gamma RIIB / CD32b >SCCHO-ATP060H

CHO/Human CD32b Stable Cell Line (High Expression) Development Service

For research use only.

描述(Description)

The CHO/Human CD32b Stable Cell Line was engineered to express full length human CD32b receptor (Gene ID: 2213), with different levels of CD32b expression (High, Medium, Low), which can be used to test agonist antibody whether in a CD32b-dependent manner to strengthen the agonistic activity. When co-cultured with Human 4-1BB HEK293 Reporter Cell and anti-4-1BB agonist antibody, the anti-4-1BB antibody can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.

应用说明(Application)

• Useful for cell-based CD32b binding assay

• Useful for CD32b-mediated crosslinking

Fc gamma RIIB / CD32b Assay Principles

生长特性(Growth Properties)

Adherent

筛选标记(Selection Marker)

Hygromycin (20 μg/mL)

培养基(Culture Medium)

F-12K+ 10% FBS

冻存液(Freeze Medium)

Serum-free cell cryopreservation medium

装量(Quantity)

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储(Storage)

Frozen in liquid nitrogen.

支原体检测(Mycoplasma Testing)

Negative

无菌检测(Sterility Testing)

Negative

使用说明(Instructions for Use)

See data sheet for detailed culturing and assay protocol.

 

Receptor Assay

Fc gamma RIIB / CD32b FACS

Expression analysis of human CD32b on CHO/Human CD32b Stable Cell Line by FACS.
Cell surface staining using PE/Cy7-labeled anti-human CD32b antibody was performed on CHO/Human CD32b Stable Cell Line with different expression levels: CHO/Human CD32b Stable Cell Line (Low Expression); CHO/Human CD32b Stable Cell Line (Medium Expression); CHO/Human CD32b Stable Cell Line (High Expression).

Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

 

License Disclosure

This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.

 

前沿进展

 
 
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+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Fc gamma RIIB / CD32b靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor II-b
中文全称:低亲和力免疫球蛋白γFc区受体II-B
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床三期
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